Purification and Properties of Carboxypeptidase A-Like Enzyme from the Starfish Asterias amurensis.
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چکیده
منابع مشابه
Asterosaponins isolated from the starfish Asterias amurensis.
Three new asterosaponins 1-3 and four known saponins 4-7 have been isolated from the starfish Asterias amurensis LÜTKEN. By means of high magnetic field 1D- and 2D-NMR ((1)H-(1)H correlation spectroscopy (COSY), total correlation spectroscopy (TOCSY), heteronuclear multiple quantum coherence (HMQC), heteronuclear single quantum coherence (HSQC), heteronuclear multiple bond correlation (HMBC), a...
متن کاملIsolation and characteristics of carboxypeptidase B from the pyloric ceca of the starfish Asterias amurensis.
Carboxypeptidase B was purified from the pyloric ceca of the starfish Asterias amurensis. The final enzyme preparation was nearly homogeneous in polyacrylamide gel electrophoresis and its molecular weight was estimated as approximately 34,000. The optimum pH and temperature of the enzyme for hydrolysis of benzoyl-glycyl-L-arginine were at approximately pH 7.5 and 55 degrees C, respectively. The...
متن کاملStarfish, Asterias amurensis and Asterina pectinifera, as potential sources of Th1 immunity-stimulating adjuvants.
Saponin is the generic name of steroid or triterpene glycosides, and the capacities of some saponins to stimulate both Th1 immune response and production of cytotoxic T cells are useful as vaccine components against intracellular pathogens. Because saponins have been found commonly in starfish, we assessed the potential of starfish, Asterias amurensis and Asterina pectinifera, as adjuvant sourc...
متن کاملPurification and properties of a carboxypeptidase inhibitor from potatoes.
* This work was supported in part by United States Public Health Service Grant 2-K3-GM 17059 (C. A. R.), United States Department of Agriculture, Cooperative States Research Service Grant 316-15-30 (C. A. R.), and bv National Institute of Health Grant GM 15731 awarded to Dr. -Hans Neurath, Department of Biochemistrv. Universitv of Washineton. $ Career Development Awardee of the United States Pu...
متن کاملCarboxypeptidase G: purification and properties.
In such substrates RCOOH can represent not only substituted pteridines but a variety of organic acids, including many amino acids. However, hydrolytic activity seems to occur only when L-glutamate with a free a-carboxyl group is the donor of the amide nitrogen in the linkage. This specificity indicates that the enzyme may be thought of as a carboxypeptidase for glutamate. An enzyme with these p...
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ژورنال
عنوان ژورنال: NIPPON SUISAN GAKKAISHI
سال: 1991
ISSN: 1349-998X,0021-5392
DOI: 10.2331/suisan.57.1939